Rare Cell Population Analysis in Early-Stage Breast Cancer Patients

Rare Cell Population Analysis in Early-Stage Breast Cancer Patients

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Background: Circulating rare cells participate in breast cancer evolution as systemic components of the disease and thus, are a source of theranostic information.Exploration of cancer-associated rare cells is in its infancy.Objectives: We aimed to investigate and classify abnormalities in the circulating rare cell population among early-stage breast cancer patients using fluorescence marker identification and cytomorphology.In addition, we sought to determine the dependency of these markers on the presence of tumors.

Design: We evaluated the validity of a multi-rare-cell detection platform and Cat Food demonstrated the utility of a specific rare cell subset as a novel approach to characterize the breast cancer system.Sampling was conducted both before and after tumor resection.Methods: Linearity of the Rarmax platform was established using a spike-in approach.The platform includes red blood cell lysis, leukocyte depletion and high-resolution fluorescence image recording.

Rare cell analysis was conducted on 28 samples (before and after surgery) from 14 patients with breast cancer, 20 healthy volunteers and 9 noncancer control volunteers.In-depth identification of rare cells, including circulating tumor cells, endothelial-like cells, erythroblasts, and inflammation-associated cells, was performed using a phenotype and morphology-based classification system.Results: The platform performed linearly over a range of 5 to 950 spiked cells, with an average recovery of 84.6%.

Circulating epithelial and endothelial-like cell subsets have been demonstrated to be associated with or independent of cancer with tumor presence.Furthermore, certain cell profile patterns may be associated with treatment-related adverse effects.The sensitivity in detecting tumor-presence and cancer-associated abnormality before surgery was 43% and 85.7%, respectively, and the specificity was 100% and 96.

6%, respectively.Conclusion: This Cleaning Supplies study supports the idea of a cancer-associated rare cell abnormality to represent tumor entities as well as systemic cancer.The latter is independent of the apparent clinical cancer.